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host strain e coli b  (ATCC)


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    ATCC host strain e coli b
    a Types and proportions of verified defense systems <t>and</t> <t>candidate</t> defense systems in cold seep microbial genomes, including their primary mechanisms of action and corresponding system counts. “DS” denotes defense systems and “num” represents the total count. “Type num” refers to the number of distinct defense system types within each mechanism category. b Plaque assays demonstrating phage infection of Escherichia <t>coli</t> <t>B</t> (ATCC ® 11303™) transformed with plasmids carrying various candidate defense systems or an empty vector (control) using a small volume drop method. The bacteria described above were mixed in the molten agar matrix and ten-fold serial dilutions of phages T7, T5, and T4 were spotted onto bacterial lawns. All the assays are repeated at least 3 times with similar results. Predicted protein domains of the tested systems are shown on the right. Detailed data on annotated defense systems are provided in Supplementary Data – .
    Host Strain E Coli B, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1791 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/host strain e coli b/product/ATCC
    Average 99 stars, based on 1791 article reviews
    host strain e coli b - by Bioz Stars, 2026-05
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    1) Product Images from "Co-occurrence of diverse defense systems shapes complex microbe-virus relationships in deep-sea cold seeps"

    Article Title: Co-occurrence of diverse defense systems shapes complex microbe-virus relationships in deep-sea cold seeps

    Journal: Nature Communications

    doi: 10.1038/s41467-025-68174-6

    a Types and proportions of verified defense systems and candidate defense systems in cold seep microbial genomes, including their primary mechanisms of action and corresponding system counts. “DS” denotes defense systems and “num” represents the total count. “Type num” refers to the number of distinct defense system types within each mechanism category. b Plaque assays demonstrating phage infection of Escherichia coli B (ATCC ® 11303™) transformed with plasmids carrying various candidate defense systems or an empty vector (control) using a small volume drop method. The bacteria described above were mixed in the molten agar matrix and ten-fold serial dilutions of phages T7, T5, and T4 were spotted onto bacterial lawns. All the assays are repeated at least 3 times with similar results. Predicted protein domains of the tested systems are shown on the right. Detailed data on annotated defense systems are provided in Supplementary Data – .
    Figure Legend Snippet: a Types and proportions of verified defense systems and candidate defense systems in cold seep microbial genomes, including their primary mechanisms of action and corresponding system counts. “DS” denotes defense systems and “num” represents the total count. “Type num” refers to the number of distinct defense system types within each mechanism category. b Plaque assays demonstrating phage infection of Escherichia coli B (ATCC ® 11303™) transformed with plasmids carrying various candidate defense systems or an empty vector (control) using a small volume drop method. The bacteria described above were mixed in the molten agar matrix and ten-fold serial dilutions of phages T7, T5, and T4 were spotted onto bacterial lawns. All the assays are repeated at least 3 times with similar results. Predicted protein domains of the tested systems are shown on the right. Detailed data on annotated defense systems are provided in Supplementary Data – .

    Techniques Used: Infection, Transformation Assay, Plasmid Preparation, Control, Bacteria



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    host strain e coli b  (ATCC)
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    ATCC host strain e coli b
    a Types and proportions of verified defense systems <t>and</t> <t>candidate</t> defense systems in cold seep microbial genomes, including their primary mechanisms of action and corresponding system counts. “DS” denotes defense systems and “num” represents the total count. “Type num” refers to the number of distinct defense system types within each mechanism category. b Plaque assays demonstrating phage infection of Escherichia <t>coli</t> <t>B</t> (ATCC ® 11303™) transformed with plasmids carrying various candidate defense systems or an empty vector (control) using a small volume drop method. The bacteria described above were mixed in the molten agar matrix and ten-fold serial dilutions of phages T7, T5, and T4 were spotted onto bacterial lawns. All the assays are repeated at least 3 times with similar results. Predicted protein domains of the tested systems are shown on the right. Detailed data on annotated defense systems are provided in Supplementary Data – .
    Host Strain E Coli B, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli host cells e coli strain b  (LGC Standards)
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    a Types and proportions of verified defense systems <t>and</t> <t>candidate</t> defense systems in cold seep microbial genomes, including their primary mechanisms of action and corresponding system counts. “DS” denotes defense systems and “num” represents the total count. “Type num” refers to the number of distinct defense system types within each mechanism category. b Plaque assays demonstrating phage infection of Escherichia <t>coli</t> <t>B</t> (ATCC ® 11303™) transformed with plasmids carrying various candidate defense systems or an empty vector (control) using a small volume drop method. The bacteria described above were mixed in the molten agar matrix and ten-fold serial dilutions of phages T7, T5, and T4 were spotted onto bacterial lawns. All the assays are repeated at least 3 times with similar results. Predicted protein domains of the tested systems are shown on the right. Detailed data on annotated defense systems are provided in Supplementary Data – .
    E Coli Host Cells E Coli Strain B, supplied by LGC Standards, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e. coli origami b (de3) host strain  (Millipore)
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    Millipore e. coli origami b (de3) host strain
    a Types and proportions of verified defense systems <t>and</t> <t>candidate</t> defense systems in cold seep microbial genomes, including their primary mechanisms of action and corresponding system counts. “DS” denotes defense systems and “num” represents the total count. “Type num” refers to the number of distinct defense system types within each mechanism category. b Plaque assays demonstrating phage infection of Escherichia <t>coli</t> <t>B</t> (ATCC ® 11303™) transformed with plasmids carrying various candidate defense systems or an empty vector (control) using a small volume drop method. The bacteria described above were mixed in the molten agar matrix and ten-fold serial dilutions of phages T7, T5, and T4 were spotted onto bacterial lawns. All the assays are repeated at least 3 times with similar results. Predicted protein domains of the tested systems are shown on the right. Detailed data on annotated defense systems are provided in Supplementary Data – .
    E. Coli Origami B (De3) Host Strain, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    reference strains b henselae atcc 49882 atcc b quintana oklahoma human isolate p brouqui e coli top10 host strain  (ATCC)
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    ATCC reference strains b henselae atcc 49882 atcc b quintana oklahoma human isolate p brouqui e coli top10 host strain
    Bacterial strains and plasmids
    Reference Strains B Henselae Atcc 49882 Atcc B Quintana Oklahoma Human Isolate P Brouqui E Coli Top10 Host Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reference strains b henselae atcc 49882 atcc b quintana oklahoma human isolate p brouqui e coli top10 host strain/product/ATCC
    Average 95 stars, based on 1 article reviews
    reference strains b henselae atcc 49882 atcc b quintana oklahoma human isolate p brouqui e coli top10 host strain - by Bioz Stars, 2026-05
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    a Types and proportions of verified defense systems and candidate defense systems in cold seep microbial genomes, including their primary mechanisms of action and corresponding system counts. “DS” denotes defense systems and “num” represents the total count. “Type num” refers to the number of distinct defense system types within each mechanism category. b Plaque assays demonstrating phage infection of Escherichia coli B (ATCC ® 11303™) transformed with plasmids carrying various candidate defense systems or an empty vector (control) using a small volume drop method. The bacteria described above were mixed in the molten agar matrix and ten-fold serial dilutions of phages T7, T5, and T4 were spotted onto bacterial lawns. All the assays are repeated at least 3 times with similar results. Predicted protein domains of the tested systems are shown on the right. Detailed data on annotated defense systems are provided in Supplementary Data – .

    Journal: Nature Communications

    Article Title: Co-occurrence of diverse defense systems shapes complex microbe-virus relationships in deep-sea cold seeps

    doi: 10.1038/s41467-025-68174-6

    Figure Lengend Snippet: a Types and proportions of verified defense systems and candidate defense systems in cold seep microbial genomes, including their primary mechanisms of action and corresponding system counts. “DS” denotes defense systems and “num” represents the total count. “Type num” refers to the number of distinct defense system types within each mechanism category. b Plaque assays demonstrating phage infection of Escherichia coli B (ATCC ® 11303™) transformed with plasmids carrying various candidate defense systems or an empty vector (control) using a small volume drop method. The bacteria described above were mixed in the molten agar matrix and ten-fold serial dilutions of phages T7, T5, and T4 were spotted onto bacterial lawns. All the assays are repeated at least 3 times with similar results. Predicted protein domains of the tested systems are shown on the right. Detailed data on annotated defense systems are provided in Supplementary Data – .

    Article Snippet: Recombinant vectors containing these candidate systems were transformed into the host strain E. coli B (ATCC ® 11303TM), which naturally lacks these systems.

    Techniques: Infection, Transformation Assay, Plasmid Preparation, Control, Bacteria

    Bacterial strains and plasmids

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Hemin-Binding Proteins as Potent Markers for Serological Diagnosis of Infections with Bartonella quintana

    doi: 10.1128/CVI.00717-12

    Figure Lengend Snippet: Bacterial strains and plasmids

    Article Snippet: B. quintana and B. henselae were cultured on Columbia agar with 5% sheep's blood (Sysmex-bioMérieux, Tokyo, Japan) for 14 days at 37°C in 5% CO 2 . table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Description a Source or reference Strains B. henselae ATCC 49882 ATCC B. quintana Oklahoma Human isolate P. Brouqui E. coli TOP10 Host strain for cloning Invitrogen E. coli BL21Star(DE3) Host strain for gene expression Invitrogen Plasmids pCR4-TOPO TA-cloning vector Invitrogen pET100D/TOPO Expression vector Invitrogen pHbpA pET100D/TOPO containing hbpA This study pHbpB pET100D/TOPO containing hbpB This study pHbpC pET100D/TOPO containing hbpC This study pHbpD pET100D/TOPO containing hbpD This study pHbpE pET100D/TOPO containing hbpE This study pHbpB1 pET100D/TOPO containing hbpB (1–354) This study pHbpB2 pET100D/TOPO containing hbpB (265–660) This study pHbpB3 pET100D/TOPO containing hbpB (601–900) This study pHbpB4 pET100D/TOPO containing hbpB (901–1362) This study pHbpD1 pET100D/TOPO containing hbpD (1–300) This study pHbpD2 pET100D/TOPO containing hbpD (151–450) This study pHbpD3 pET100D/TOPO containing hbpD (301–600) This study pHbpD4 pET100D/TOPO containing hbpD (451–750) This study pHbpD5 pET100D/TOPO containing hbpD (601–885) This study Open in a separate window a Numbers indicate the positions in the nucleotide sequence of the appropriate gene.

    Techniques: Plasmid Preparation, Cloning, Gene Expression, Expressing

    Western blots of sera from homeless people with HbpD of B. quintana. (A) Western blots of B. henselae and B. quintana with sera from homeless people. Whole-cell lysates of B. henselae (lanes 1) and B. quintana (lanes 2) were separated on a 12.5% SDS-PAGE gel and subjected to Western blotting with five sera (top). IgG titers for Bartonella, measured with a Bartonella IFA IgG kit, are indicated under the serum numbers. Asterisks indicate a protein of approximately 35 kDa in B. quintana lysate that reacts strongly to these sera. (B) E. coli lysates expressing Xpress-tagged HbpD were subjected to Western blotting with anti-Xpress or sera from homeless people as indicated at the top.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Hemin-Binding Proteins as Potent Markers for Serological Diagnosis of Infections with Bartonella quintana

    doi: 10.1128/CVI.00717-12

    Figure Lengend Snippet: Western blots of sera from homeless people with HbpD of B. quintana. (A) Western blots of B. henselae and B. quintana with sera from homeless people. Whole-cell lysates of B. henselae (lanes 1) and B. quintana (lanes 2) were separated on a 12.5% SDS-PAGE gel and subjected to Western blotting with five sera (top). IgG titers for Bartonella, measured with a Bartonella IFA IgG kit, are indicated under the serum numbers. Asterisks indicate a protein of approximately 35 kDa in B. quintana lysate that reacts strongly to these sera. (B) E. coli lysates expressing Xpress-tagged HbpD were subjected to Western blotting with anti-Xpress or sera from homeless people as indicated at the top.

    Article Snippet: B. quintana and B. henselae were cultured on Columbia agar with 5% sheep's blood (Sysmex-bioMérieux, Tokyo, Japan) for 14 days at 37°C in 5% CO 2 . table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Description a Source or reference Strains B. henselae ATCC 49882 ATCC B. quintana Oklahoma Human isolate P. Brouqui E. coli TOP10 Host strain for cloning Invitrogen E. coli BL21Star(DE3) Host strain for gene expression Invitrogen Plasmids pCR4-TOPO TA-cloning vector Invitrogen pET100D/TOPO Expression vector Invitrogen pHbpA pET100D/TOPO containing hbpA This study pHbpB pET100D/TOPO containing hbpB This study pHbpC pET100D/TOPO containing hbpC This study pHbpD pET100D/TOPO containing hbpD This study pHbpE pET100D/TOPO containing hbpE This study pHbpB1 pET100D/TOPO containing hbpB (1–354) This study pHbpB2 pET100D/TOPO containing hbpB (265–660) This study pHbpB3 pET100D/TOPO containing hbpB (601–900) This study pHbpB4 pET100D/TOPO containing hbpB (901–1362) This study pHbpD1 pET100D/TOPO containing hbpD (1–300) This study pHbpD2 pET100D/TOPO containing hbpD (151–450) This study pHbpD3 pET100D/TOPO containing hbpD (301–600) This study pHbpD4 pET100D/TOPO containing hbpD (451–750) This study pHbpD5 pET100D/TOPO containing hbpD (601–885) This study Open in a separate window a Numbers indicate the positions in the nucleotide sequence of the appropriate gene.

    Techniques: Western Blot, SDS Page, Expressing